Genetics

Publications since 1987

2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1990 1989 1988 1987

2008

  • Thoms, B., Borchers, I. Wackernagel, W. (2008) Effects of single-strand DNases ExoI, RecJ, ExoVII, and SbcCD on homologous recombination of recBCD+ strains of Escherichia coli and roles of SbcB15 and XonA2 mutant enzymes of ExoI. J. Bacteriol. 190, 179-192.
  • Hülter, N., Wackernagel, W. (2008) Double illegitimate recombination events integrate DNA segments through two different mechanisms during natural transformation of Acinetobacter baylyi. Mol. Microbiol. 67, 984-995.
  • Harms, K., Wackernagel, W. (2008) The RecBCD and SbcCD DNases suppress homology-facilitated illegitimate recombination during natural transformation of Acinetobacter baylyi. (submitted)

2007

  • Harms, K., de Vries, J., Wackernagel, W. (2007) A double kill gene cassette for the positive selection of transforming non-selective DNA segments in Acinetobacter baylyi BD413. J. Microbiol. Methods 69, 107-115.
  • Kickstein, E., Harms, K., Wackernagel, W. (2007) recBCD and recD defects influence genetic transformation differently and are lethal together with recJ deficiency in Acinetobacter baylyi. Microbiology 153, 2259-2270.
  • Harms, K., Schön, V., Kickstein, E. and Wackernagel, W. (2007) The RecJ DNase strongly suppresses genomic integration of short but not long foreign DNA fragments by homology-facilitated illegitimate recombination during transformation of Acinetobacter baylyi. Mol. Microbiol. 64, 691-702.

2006

  • Wackernagel, W. (2006) The various sources and the fate of nucleic acids in soil. In: P. Nannipieri and K. Smalla (eds.) Soil Biology, Vol. 8, Nucleic Acids and Proteins in Soil, Springer-Verlag, Berlin Heidelberg, pp. 117-139.

2005

  • Sikorski, J., Lalucat, J., Wackernagel, W. (2005) Genomovars 11 to 18 of Pseudomonas stutzeri, identified among isolates from soil and marine sediment. IJSEM (Int. J. Syst. Evol. Microbiol.) 55, 1767-1770.
  • de Vries, J., Wackernagel, W. (2005) Microbial horizontal gene transfer and the DNA release from transgenic crop plants. Plant Soil 266: 91-104.
    [Abstract] [PDF] [Full text] [Reprint]
  • Meier, P., Wackernagel, W. (2005) Impact of mutS inactivation on foreign DNA acquisition by natural transformation in Pseudomonas stutzeri. J Bacteriol 187: 143-154.
    [Abstract] [PDF] [Full text] [Reprint]

2004

  • de Vries, J., Herzfeld, T., Wackernagel, W. (2004) Transfer of plastid DNA from tobacco to the soil bacterium Acinetobacter sp. by natural transformation. Mol Microbiol 53: 323-334.
    [Abstract] [PDF] [Full text] [Reprint]
  • Van den Eede, G., Aarts, H., Buhk, H.-J., Corthier, G., Flint, H. J., Hammes, W., Jacobsen, B., Midtvedt, T., van der Vossen, J., von Wright, A., Wackernagel, W., Wilcks, A. (2004) The relevance of gene transfer to the safety of food and feed derived from genetically modified (GM) plants. Food and Chemical Toxicology 42: 1127-1156.
    [Abstract] [PDF] [Full text] [Reprint]
  • Wackernagel, W. (2004) Environmental effects of a T4 lysozyme transgenic potato plant: Issues, analyses and conclusions. In: Risk hazard damage - Specification of criteria to assess environmental impact of genetically modified organisms (B. Breckling, R. Verhoeven, eds.), Bonn (Bundesamt für Naturschutz), Naturschutz und biologische Vielfalt 1: 25-35.
    [Abstract] [PDF] [Full text] [Reprint]
  • Wackernagel, W. (2004) The various sources and the fate of nucleic acids in soil. In: Soil Biology. (P. Nannipieri, K. Smalla, eds.), Springer-Verlag, Heidelberg. in press.
    [Abstract] [PDF] [Full text] [Reprint]

2003

  • de Vries, J., Heine, M., Harms, K., Wackernagel, W. (2003) Spread of recombinant DNA by roots and pollen of transgenic potato plants, identified by highly specific biomonitoring using natural transformation of an Acinetobacter sp. Appl Environ Microbiol 69: 4455-4462.
    [Abstract] [PDF] [Full text] [Reprint]
  • Meier, P., Wackernagel, W. (2003) Monitoring the spread of recombinant DNA from field plots with transgenic sugar beet plants by PCR and natural transformation of Pseudomonas stutzeri. Transgenic Res 12: 293-304.
    [Abstract] [PDF] [Full text] [Reprint]
  • Berndt, C., Meier, P., Wackernagel, W. (2003) DNA restriction is a barrier to natural transformation in Pseudomonas stutzeri JM300. Microbiology 149: 895-901.
    [Abstract] [PDF] [Full text] [Reprint]
  • Meier, P., Wackernagel, W. (2003) Mechanisms of homology-facilitated illegitimate recombination for foreign DNA acquisition in transformable Pseudomonas stutzeri. Mol Microbiol 48: 1107-1118.
    [Abstract] [PDF] [Full text] [Reprint]
  • Wackernagel, W., Düring, K. (2003) Transgenic potato plants expressing anti-microbial activity: establishment of pest control and environmental safety. In: Modern Aspects of Nutrition. Present Knowledge and Future Perspectives. (I. Elmadfa, E. Anklam, J. S. König, eds.) Forum Nutr. Vol. 56, pp. 317-318. Karger Verlag, Basel, Schweiz.
    [Abstract] [PDF] [Full text] [Reprint]

2002

  • de Vries, J., Wackernagel, W. (2002) Integration of foreign DNA during natural transformation of Acinetobacter sp. by homology-facilitated illegitimate recombination. Proc Natl Acad Sci USA 99: 2094-2099.
    [Abstract] [PDF] [Full text] [Reprint]
  • Sikorski, J., Teschner, N. Wackernagel, W. (2002) Highly different levels of natural transformation are associated with genomic subgroups within a local population of Pseudomonas stutzeri from soil. Appl Environ Microbiol 68: 865-873.
    [Abstract] [PDF] [Full text] [Reprint]
  • Meier, P., Berndt, C., Weger, N. Wackernagel, W. (2002) Natural transformation of Pseudomonas stutzeri by single-stranded DNA requires type IV pili, competence state and comA. FEMS Microbiol Lett 207: 75-80.
    [Abstract] [PDF] [Full text] [Reprint]
  • Sikorski, J., Möhle, M., Wackernagel, W. (2002) Identification of complex composition, strong strain diversity and directional selection in local Pseudomonas stutzeri populations from marine sediment and soils. Environ Microbiol 4: 465-476.
    [Abstract] [PDF] [Full text] [Reprint]

2001

  • de Vries, J., Meier, P., Wackernagel, W. (2001) The natural transformation of the soil bacteria Pseudomonas stutzeri and Acinetobacter sp. by transgenic plant DNA strictly depends on homologous sequences in the recipient cells. FEMS Microbiol Lett 195: 211-215.
    [Abstract] [PDF] [Full text] [Reprint]
  • Wackernagel, W., de Vries, J. (2001) Transformation von Bakterien durch Antibiotikum-Resitenzgene aus transgenen Pflanzen und Nahrungsmitteln. Ernährungsforschung 46: 1-12.
    [Abstract] [PDF] [Full text] [Reprint]
  • Graupner, S., Weger, N., Sohni, M., Wackernagel, W. (2001) Requirement of novel competence genes pilT and pilU of Pseudomonas stutzeri for natural transformation and suppression of pilT deficiency by a hexahistidine tag on the type IV pilus protein PilAI. J Bacteriol 183: 4694-4701.
    [Abstract] [PDF] [Full text] [Reprint]
  • Jonas, D.A., Elmadfa, I., Engel, K.H., Heller, K.J., Kozianowski, G., Konig, A., Muller, D., Narbonne, J.F., Wackernagel, W., Kleiner, J. (2001) Safety considerations of DNA in food. Ann Nutr Metab 45:235-254.
    [Abstract] [PDF] [Full text] [Reprint]
  • Sikorski, J., Jahr, H., Wackernagel, W. (2001) The structure of a local population of phytopathogenic Pseudomonas brassicacearum from agricultural soil indicates development under purifying selection pressure. Environ Microbiol 3: 176-186.
    [Abstract] [PDF] [Full text] [Reprint]
  • Graupner, S., Wackernagel, W. (2001) Pseudomonas stutzeri has two closely related pilA genes (type IV pilus structural protein) with opposite influences on natural genetic transformation. J Bacteriol 183: 2359-2366.
    [Abstract] [PDF] [Full text] [Reprint]
  • Graupner, S., Wackernagel, W. (2001) Identification and characterization of novel competence genes comA and exbB involved in natural genetic transformation of Pseudomonas stutzeri. Res Microbiol 152: 451-460.
    [Abstract] [PDF] [Full text] [Reprint]

2000

  • Ahrenholtz, I., Harms, K., de Vries, J., Wackernagel, W. (2000) Increased killing of Bacillus subtilis on the hair roots of transgenic T4 lysozyme producing potatoes. Appl Environ Microbiol 66: 1862-1865.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lottmann, J., Heuer, H., de Vries, J., Mahn, A., Düring, K., Wackernagel, W., Smalla, K., Berg, G. (2000) Establishment of introduced antagonistic bacteria in the rhizosphere of transgenic potatoes and their effect on the bacterial community. FEMS Microbiol Ecol 33: 41-49.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Sikorski, J. (2000) The potential for intraspecific horizontal gene exchange by natural genetic transformation: sexual isolation among genomovars of Pseudomonas stutzeri. Microbiology 146: 3071-3080.
    [Abstract] [PDF] [Full text] [Reprint]
  • Graupner, S., Wackernagel, W. (2000) A broad-host-range expression vector series including a Ptac test plasmid and its application in the expression of the dod gene of Serratia marcescens (coding for ribulose-5-phosphate 3-epimerase) in Pseudomonas stutzeri. Genetic Analysis: Biomolecular Engineering 17: 11-16.
    [Abstract] [PDF] [Full text] [Reprint]
  • Graupner, S., Frey, V., Hashemi, R., Lorenz, M.G., Brandes, G., Wackernagel, W. (2000) Type IV pilus genes pilA and pilC of Pseudomonas stutzeri are required for natural genetic transformation, and pilA can be replaced by corresponding genes from nontransformable species. J Bacteriol 182: 2184-2190.
    [Abstract] [PDF] [Full text] [Reprint]

1999

  • Ostendorf, T., Cherepanov, P., de Vries, J., Wackernagel, W. (1999) Characterization of a dam mutant of Serratia marcescens and the nucleotide sequence of the dam region. J Bacteriol 181: 3880-3885.
    [Abstract] [PDF] [Full text] [Reprint]
  • de Vries, J., Harms, K., Broer, I., Kriete, G., Mahn, A., Düring, K., Wackernagel, W. (1999) The bacteriolytic activity in transgenic potatoes expressing a chimeric T4 lysozyme gene and the effect of T4 lysozyme on soil- and phytopathogenic bacteria. System Appl Microbiol 22: 280-286.
    [Abstract] [PDF] [Full text] [Reprint]
  • Sikorski, J., Rosselló-Mora, R., Lorenz, M.G. (1999) Analysis of genotypic diversity and relationships among Pseudomonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis. System Appl Microbiol 22: 393-402.
    [Abstract] [PDF] [Full text] [Reprint]

1998

  • de Vries, J., Wackernagel, W. (1998) Detection of nptII (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation. Mol Gen Genet 257: 606-613.
    [Abstract] [PDF] [Full text] [Reprint]
  • Sikorski, J., Graupner, S., Lorenz, M.G., Wackernagel, W. (1998) Natural genetic transformation of Pseudomonas stutzeri in a nonsterile soil. Microbiology 144: 569-576.
    [Abstract] [PDF] [Full text] [Reprint]
  • Thoms, B., Wackernagel, W. (1998) Interaction of RecBCD enzyme with DNA at double-strand breaks produced in UV-irradiated Escherichia coli: Requirement for DNA end processing. J Bacteriol 180: 5639-5645.
    [Abstract] [PDF] [Full text] [Reprint]

1997

  • Blum, S.A.E., Lorenz, M.G., Wackernagel, W. (1997) Mechanism of retarded DNA degradation and prokaryotic origin of DNases in nonsterile soil. System Appl Microbiol 20: 513-521.
    [Abstract] [PDF] [Full text] [Reprint]
  • Wackernagel, W., Sikorski, J., Blum, S.A.E., Lorenz, M.G., Graupner, S. (1997) Natural genetic transformation of bacteria in soil. In: Horizontal Gene Transfer (M. Syvanen and C. Kado, eds.).
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Meyer, B., Wittstock, M., Graupner, S., Wackernagel, W. (1997) Selective DNA uptake and DNA restriction as barriers to horizontal gene exchange by natural genetic transformation in Pseudomonas stutzeri JM300. In: Horizontal Gene Transfer (M. Syvanen, C. Kado, eds.).
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G. (1997) Horizontal gene transfer among bacteria in soil by natural genetic transformation. In: Microbial interactions in agriculture and forestry. N.S. Subbarao, Y.R. Dommergues (eds.) Oxford and IBH Publishing Co., New Delhi.
    [Abstract] [PDF] [Full text] [Reprint]
  • de Vries, J., Wackernagel, W. (1997) Wandernde Gene. Einblicke 26: 12-14.
    [Abstract] [PDF] [Full text] [Reprint]

1996

  • Wackernagel, W. (1996) The persistence of DNA in the environment and its potential for bacterial genetic transformation. In: E.R. Schmidt, T. Hankeln (eds.)Transgenic Organisms and Biosafety. Horizontal Gene Transfer, Stability of DNA and Expression of Transgenes. Springer-Verlag, Heidelberg, Germany, pp 137-146.
    [Abstract] [PDF] [Full text] [Reprint]
  • Blum, S.A.E., Lorenz, M.G., Wackernagel, W. (1996) Persistence of DNA in natural soils: Adsorption to particulate material provides protection against nucleolytic degradation. In: E.R. Schmidt, T. Hankeln (eds.):Transgenic Organisms and Biosafety. Horizontal Gene Transfer, Stability of DNA and Expression of Transgenes. Springer-Verlag, Heidelberg, Germany, pp 147-154.
    [Abstract] [PDF] [Full text] [Reprint]
  • Jekel, M., Lorenz, M.G., Wackernagel, W. (1996) A conditional suicide system for genetically engineered bacteria based on the endonuclease I gene of Escherichia coli. In: E.R. Schmidt, T. Hankeln (eds.): Transgenic Organisms and Biosafety. Horizontal Gene Transfer, Stability of DNA and Expression of Transgenes. Springer-Verlag, Heidelberg, Germany, pp 165-169.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Meyer, B., Wackernagel, W. (1996) Efficiency of horizontal transfer by natural genetic transformation of plasmids with homology to the Pseudomonas stutzeri chromosome and the effect of DNA restriction. In: E.R. Schmidt, T. Hankeln (eds.):Transgenic Organisms and Biosafety. Horizontal Gene Transfer, Stability of DNA and Expression of Transgenes. Springer-Verlag, Heidelberg, Germany, pp 101-104.
    [Abstract] [PDF] [Full text] [Reprint]
  • Graupner, S., Wackernagel, W. (1996) Identification and characterization of extrachromosomal circular DNA released from a genetically modified chromosome of Hansenula polymorpha. In: E.R. Schmidt, T. Hankeln (eds.):Transgenic Organisms and Biosafety. Horizontal Gene Transfer, Stability of DNA and Expression of Transgenes. Springer-Verlag, Heidelberg, Germany, pp 171-180.
    [Abstract] [PDF] [Full text] [Reprint]
  • Ahrenholtz, I., Lorenz, M.G., Wackernagel, W. (1996) Degradation of transforming DNA in a groundwater aquifer microcosm by the extracellular nuclease of Serratia marcescens SM6 and application of the nuclease in a new conditional suicide system for Escherichia coli. In: E.R. Schmidt, T. Hankeln (eds.): Transgenic Organisms and Biosafety. Horizontal Gene Transfer, Stability of DNA and Expression of Transgenes. Springer-Verlag, Heidelberg, Germany, pp 155-163.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Wackernagel, W. (1996) Mechanisms and consequences of horizontal gene transfer in natural bacterial populations. In: J. Tomiuk, A. Sentker, W. Wöhrmann (eds.):Transgenic Organisms. Biological and Social Implications. Birkhäuser-Verlag, Basel, Boston, Berlin, pp 45-57.
    [Abstract] [PDF] [Full text] [Reprint]
  • Graupner, S., Wackernagel, W. (1996) Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of Escherichia coli. Appl Environ Microbiol 62: 1839-1841.
    [Abstract] [PDF] [Full text] [Reprint]
  • Mäkelä, P.H., Braun, R., Alexieva, Z.M., Bruni, C.B., Oxelfeldt, P., Schofield, M., Teuber, M., Tomcsanyi, T., Wackernagel, W. (1995) Gene Technology in Microbiology: Benefits and Risks. Fed. Europ. Microb. Soc. (FEMS) Report 1996, pp 1-16.
    [Abstract] [PDF] [Full text] [Reprint]

1995

  • Jekel, M., Wackernagel, W. (1995) The periplasmic endonuclease I of Escherichia coli has amino acid sequence homology to the extracellular DNases of Vibrio cholerae and Aeromonas hydrophila. Gene 154: 55-59.
    [Abstract] [PDF] [Full text] [Reprint]
  • Cherepanov, P.P., Wackernagel, W. (1995) Gene disruption in Escherichia coli: TcR and KmR cassettes with an option of FLP-catalyzed excision of the antibiotic resistance determinant. Gene 158: 9-14.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Wackernagel, W. (1995) Die Rolle der Sexualität in der Evolution von Prokaryoten. BioSpektrum 1: 28-33.
    [Abstract] [PDF] [Full text] [Reprint]
  • Köppen, A., Krobitsch, S., Thoms, B., Wackernagel, W. (1995) Interaction with the recombination hot spot Chi in vivo converts the RecBCD enzyme of Escherichia coli into a Chi-independent recombinase by inactivation of the RecD subunit. Proc Natl Acad Sci USA 92: 6249-6253.
    [Abstract] [PDF] [Full text] [Reprint]

1994

  • de Vries, J., Genschel, J., Urbanke, C., Thole, H., Wackernagel, W. (1994) The single-stranded-DNA-binding proteins (SSB) of Proteus mirabilis and Serratia marcescens. Eur J Biochem 224: 613-622.
    [Abstract] [PDF] [Full text] [Reprint]
  • de Vries, J., Wackernagel, W. (1994) Cloning and sequencing of the Proteus mirabilis gene for a single-stranded DNA binding protein (SSB) and complementation of Escherichia coli ssb point and deletion mutations. Microbiology 140: 889-895.
    [Abstract] [PDF] [Full text] [Reprint]
  • Ahrenholtz, I., Lorenz, M.G., Wackernagel, W. (1994) The extracellular nuclease of Serratia marcescens: studies on the activity in vitro and effect on transforming DNA in a groundwater aquifer microcosm. Arch Microbiol 161: 176-183.
    [Abstract] [PDF] [Full text] [Reprint]
  • Jekel, M., Wackernagel, W. (1994) Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 genome. J Bacteriol , 176: 1550-1551.
    [Abstract] [PDF] [Full text] [Reprint]
  • Wackernagel, W., Lorenz, M.G. (1994) DNA-Entlassung aus Bakterien, DNA-Überdauerung und genetische Transformation im natürlichen Lebensraum. In: Biologische Sicherheit , Band III, 9-33.
    [Abstract] [PDF] [Full text] [Reprint]
  • Wackernagel, W. (1994) Gentransfer bei Bakterien und Verhalten gentechnisch veränderter Bakterien im Boden und Wasser bei Freisetzungssimulierung. In: Biologische Sicherheit, Band III, 1-7.
    [Abstract] [PDF] [Full text] [Reprint]
  • Wackernagel, W., Lorenz, M.G. (1994) Nachweis von Mikroorganismen und DNA in Böden. In:Überwachungsmethoden Gentechnik: Nachweisverfahren für Mikroorganismen, Viren und Gene in der Umwelt. Reihe UBA-Texte, Berlin, 259-279.
    [Abstract] [PDF] [Full text] [Reprint]
  • Romanowski, G., Lorenz, M.G., Wackernagel, W. (1994) Monitoring der Überdauerung extrazellulärer Plasmid-DNA in Böden durch PCR und genetische Transformation. In: Überwachungsmethoden Gentechnik: Nachweisverfahren für Mikroorganismen, Viren und Gene in der Umwelt. Reihe UBA-Texte, Berlin, 447-454.
    [Abstract] [PDF] [Full text] [Reprint]
  • Basse, G., Lorenz, M.G., Wackernagel, W. (1994) A biological assay for the sensitive and quantitative detection of extracellular microbial DNases. J Microbiol Methods 20: 137-147.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Wackernagel, W. (1994) Bacterial gene transfer by natural genetic transformation in the environment. Microbiol Rev 58: 563-602.
    [Abstract] [PDF] [Full text] [Reprint]
  • Ahrenholtz, I., Lorenz, M.G., Wackernagel, W. (1994) A conditional suicide system in Escherichia coli based on the intracellular degradation of DNA. Appl Environ Microbiol 60: 3746-3751.
    [Abstract] [PDF] [Full text] [Reprint]
  • Tebbe, C., Vahjen, W., Munch, J.C., Meier, B., Gellissen, G., Feldmann, S., Sahm, H., Amore, R., Hollenberg, C., Blum, S., Wackernagel, W. (1994) Mesokosmenuntersuchungen und Einfluß der Habitatbedingungen auf die Expression, Überdauerung und Übertragung des Aprotinin-Gens. BioEngineering 6: 22-26.
    [Abstract] [PDF] [Full text] [Reprint]

1993

  • de Vries, J., Wackernagel, W. (1993) Cloning and sequencing of the Serratia marcescens gene encoding a single-stranded DNA-binding protein (SSB) and its promoter region. Gene 127: 39-45.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Wackernagel, W (1993). Bacterial gene transfer in the environment. In: Transgenic organisms. Risk assessment of deliberate releases.(W. Wöhrmann, J. Tomiuk, eds.) Birkhäuser (Basel, Boston, Berlin), pp. 43-64.
    [Abstract] [PDF] [Full text] [Reprint]
  • Chamier, B., Lorenz, M.G., Wackernagel, W. (1993) Natural transformation of Acinetobacter calcoaceticus by plasmid DNA adsorbed on sand and groundwater aquifer material. Appl Environ Microbiol 59: 1662-1667.
    [Abstract] [PDF] [Full text] [Reprint]
  • Romanowski, G., Lorenz, M.G., Wackernagel, W. (1993) Plasmid DNA in a groundwater aquifer microcosm: adsorption, DNase resistance and natural genetic transformation of Bacillus subtilis. Molec Ecol 2: 171-181.
    [Abstract] [PDF] [Full text] [Reprint]
  • Romanowski, G., Lorenz, M.G., Wackernagel, W. (1993) Use of polymerase chain reaction and electroporation of Escherichia coli to monitor the persistence of extracellular plasmid DNA introduced into natural soils. Appl Environ Microbiol 59: 3438-3446.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Wackernagel, W (1993). Transformation as a mechanism for bacterial gene transfer in soil and sediment - studies with a sand/clay microcosm and the Cyanobacterium Synechocystis OL50. In: Trends in Microbial Ecology (R. Guerrero, C. Pedros-Alio, eds.), 325-330, Spanish Society for Microbiology.
    [Abstract] [PDF] [Full text] [Reprint]

1992

  • de Vries, J., Wackernagel, W. (1992) Recombination and UV resistance of Escherichia coli with the cloned recA and recBCD genes of Serratia marcescens and Proteus mirabilis: evidence for an advantage of intraspecies combination of P. mirabilis RecA protein and RecBCD enzyme. J Gen Microbiol 138: 31-38.
    [Abstract] [PDF] [Full text] [Reprint]
  • Katzek, J. und Wackernagel, W. (1992) Stand der Forschung im Bereich der Risikoabschätzung beim Umgang mit gentechnisch veränderten Organismen und Aufdeckung von Forschungsdefiziten. Hans-Böckler-Stiftung (Herausgeber), Bochum, 210 Seiten.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Reipschläger, K., Wackernagel, W. (1992) Plasmid transformation of naturally competent Acinetobacter calcoaceticus in non-sterile soil extract and groundwater. Arch Microbiol 157: 355-360.
    [Abstract] [PDF] [Full text] [Reprint]
  • Rinken, R., Wackernagel, W. (1992) Inhibition of the recBCD-dependent activation of Chi recombinational hotspots on SOS-induced cells of Escherichia coli. J Bacteriol 174: 1172-1178.
    [Abstract] [PDF] [Full text] [Reprint]
  • Wackernagel, W., Romanowski, G., Lorenz, M.G. (1992) Studies on gene flux by free bacterial DNA in soil, sediment and groundwater aquifer. In: The release of genetically engineered microorganisms. (D.E.S. Stewart-Tull, M. Sussman, eds.) Plenum Publ. Corp., New York, 171-174.
    [Abstract] [PDF] [Full text] [Reprint]
  • Rinken, R., Thoms, B., Wackernagel, W. (1992) Evidence that the recBC-dependent degradation of duplex DNA in recD mutants of Escherichia coli involves DNA unwinding. J Bacteriol 174: 5424-5429.
    [Abstract] [PDF] [Full text] [Reprint]
  • Romanowski, G., Lorenz, M.G., Sayler, G., Wackernagel, W. (1992) Persistence of free plasmid DNA in soil monitored by various methods, including a transformation assay. Appl Environ Microbiol 58: 3012-3019.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Wackernagel, W. (1992) Stimulation of natural genetic transformation of Pseudomonas stutzeri in extracts from various soils by nitrogen or phosphorus limitation and influence of temperature and pH. Microbial Releases 1: 173-176.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G., Wackernagel, W. (1992) DNA binding to various clay minerals and retarded enzymatic degradation of DNA in a sand/clay microcosm. In: Gene transfers and environment (M.J. Gauthier, ed.) Springer (Berlin, Heidelberg, New York) pp. 103-113.
    [Abstract] [PDF] [Full text] [Reprint]
  • Bruns, S., Reipschläger, K., Lorenz, M.G., Wackernagel, W. (1992) Characterization of natural transformation of the soil bacteria Pseudomonas stutzeri and Acinetobacter calcoaceticus by chromosomal and plasmid DNA. In: Gene transfers and environment (M.J. Gauthier, ed.) Springer (Berlin, Heidelberg, New York) 115-126.
    [Abstract] [PDF] [Full text] [Reprint]
  • Lorenz, M.G. (1992) Gene transfer via transformation in soil/sediment environments. In: Gene transfers and environment. M.J. Gauthier (ed.), Springer Verlag, Berlin, pp. 95-101.
    [Abstract] [PDF] [Full text] [Reprint]

1991

  • Rinken, R., de Vries, J., Weichenhan, D., Wackernagel, W. (1991) The recA-recBCD dependent recombination pathways of Serratia marcescens and Proteus mirabilis in Escherichia coli: functions of hybrid enzymes and hybrid pathways. Biochimie 73: 375-384.
    [Abstract] [PDF] [Full text] [Reprint]
  • Romanowski, G., Lorenz, M.G., Wackernagel, W. (1991) Adsorption of plasmid DNA to mineral surfaces and protection against DNaseI. Appl Environ Microbiol 57: 1057-1061.
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  • Lorenz, M.G., Wackernagel, W. (1991) High frequency of natural genetic transformation of Pseudomonas stutzeri in soil extract supplied with a carbon/energy and a phosphorus source. Appl Environ Microbiol 57: 1246-1251.
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  • Lorenz, M.G., Gerjets, D., Wackernagel, W. (1991) Release of transforming plasmid and chromosomal DNA from two cultured soil bacteria. Arch Microbiol 156: 319-326.
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1990

  • Wackernagel, W. (1990) Zur Bedeutung des bakteriellen Gentransfers durch extrazelluläres Erbmaterial im Freiland für die Freisetzung gentechnisch veränderter Bakterien. In: Die Zukunft der Nutzpflanzen (S. Albrecht, Hg.), Gentechnologie: Chancen und Risiken, Band 24: 117-126, Campus Verlag, Frankfurt/New York,.
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  • Romanowski, G., Lorenz, M.G., Wackernagel, W. (1990) Bakterieller Gentransfer durch freie DNA im natürlichen Lebensraum: Studien an einem Bodenmodell. In: Biologische Sicherheit (BMFT, Hg.), 11-26.
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  • Lorenz, M.G., Wackernagel, W. (1990) Natural genetic transformation of Pseudomonas stutzeri by sand-adsorbed DNA. Arch Microbiol , 154: 380-385.
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  • Wackernagel, W. (1990) Aspekte der Freisetzung gentechnisch veränderter Mikroorganismen. In: Gentechnik in Biotechnologie, Land- und Forstwirtschaft (Bundesminister für Land- und Forstwirtschaft, Hg.), Wien, 86-97.
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1989

  • Wackernagel, W., Rinken, R., Weichenhan, D. (1989) Recombination and repair in Escherichia coli by recBCD enzymes from Proteus mirabilis and Serratia marcescens: functional analysis of heterologous, of hybrid, and of mutant enzymes. J Cell Biochem, Suppl. 13D, 113.
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  • Weichenhan, D., Wackernagel, W. (1989) Functional analyses of Proteus mirabilis wildtype and mutant recBCD enzymes in Escherichia coli reveal a new mutant phenotype. Mol Microbiol 3: 1777-1784.
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  • Wackernagel, W. (1989) Strahlenbiologie. In: Trends der Modernen Biologie. (U. Winkler, Hg), 63-70, Stuttgart.
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  • Lorenz, M.G., Wackernagel, W. (1989) Postmortale Vererbung bei Bakterien. Labor 2000 (Dez.), 30-34, Vogel-Verlag, Würzburg.
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  • Wackernagel, W (1989). Die Erbsubstanz als Ausgangspunkt und Ziel von zellulären Lebensvorgängen. Einblicke 9: 28-31.
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1988

  • Lorenz, M.G., Aardema, B.W., Wackernagel, W. (1988) Highly efficient genetic transformation of Bacillus subtilis attached to sand grains. J Gen Microbiol 134: 107-112.
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  • Thoms, B., Wackernagel, W. (1988) Suppression of the postreplication repair deficiency of recF mutants of Escherichia coli by recA(Srf) and recA(Tif) depends on the recJ function. J Cell Biochem, Suppl. 12A,.
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  • Lorenz, M.G., Wackernagel, W. (1988) Impact of mineral surfaces on gene transfer by transformation in natural bacterial environments. In: Risk assessment for deliberate releases. (W. Klingmüller, ed.), pp. 110-119, Springer Verlag.
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  • Weichenhan, D., Wackernagel, W. (1988) Cloning of the recB, recC, and recD genes from Proteus mirabilis in Escherichia coli: in vivo formation of active hybrid enzymes. J Bacteriol 170: 1412-1414.
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  • Thoms, B., Wackernagel, W. (1988) Suppression of the UV-sensitive phenotype of Escherichia coli recF mutants by recA(Srf) and recA(Tif) mutations requires recJ+. J Bacteriol 170: 3675-3681.
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  • Wackernagel, W. (1988) Molekulargenetik von Prokaryoten. BioEngineering 4: 219-221.
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  • Lorenz, M.G., Wackernagel, W. (1988) Gentransfer bei Bakterien und Verhalten gentechnisch veränderter Bakterien im Boden- und Grundwasserbereich: DNA-Freisetzung aus Zellen, DNA-Überdauerung und genetische Transformation im natürlichen Lebensraum. In: Biologische Sicherheit (BMFT, Herausgeber), Bonn, pp. 129-141.
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  • Filip, Z., Klingmüller, W., Wackernagel, W. (1988) Untersuchungen zum Verhalten genmanipulierter Bakterien im Boden und Grundwasser. Bundesgesundheitsblatt 31: 432-434.
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1987

  • Romanowski, G., Weichenhan, D., Gram, M., Wackernagel, W. (1987) Effect of recBCD enzyme overproduction in Escherichia coli on recombination, repair of UV damage and propagation of phages Lambda, T7 and T4. Mol Gen (Life Sci Adv) 6: 71-74.
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  • Thoms, B., Wackernagel, W. (1987) Regulatory role of recF in the SOS response of Escherichia coli: Impaired induction of SOS genes by UV irradiation and nalidixic acid in a recF mutant. J Bacteriol 169: 1731-1736.
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  • Lorenz. M.G., Wackernagel, W. (1987) Adsorption of DNA to sand and variable degradation rates of adsorbed DNA. Appl Environ Microbiol 53: 2948-2952.
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  • Weichenhan, D., Wackernagel, W. (1987) The function in genetic recombination and DNA repair of recBCD enzymes composed in vivo of subunits from Escherichia coli and Proteus mirabilis. BioEngineering Suppl. 2: 119.
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  • Lorenz, M.G., Wackernagel, W. (1987) A model of gene transfer in soil and sediments: Retarded degradation of DNA and genetic transformation at the surface of sand grains. BioEngineering Suppl. 2: 120.
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  • Lorenz, M.G., Wackernagel, W. (1987) Gene transfer in the natural environment: Genetic transformation at the surface of sand grains. In: Genetic flux and horizontal evolution in bacteria. (J.-P. Gratia, ed.) pp. 38-39, University Press, Brüssel.
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